Project

Part:BBa_K1419005

Designed by: Harland Brandon   Group: iGEM14_Lethbridge   (2014-10-09)

RVG-Lamp2B-Clover

This part contains the Lamp2B coding sequence with a rabies virus glycoprotein tag and in-fusion clover (GFP) sequence for easy localization of Lamp2B C-terminal modification.

This part was used for determining the localization of C-terminal additions to Lamp2B. Clover takes the place of a DNA binding protein that will be used to target plasmids to the exosomal interior.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 119
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 153


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Parameters
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